NIH/3T3-Luc cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

NIH/3T3-Luc Order

Catalog#

YC-A013-Luc-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Luciferase Stable Cell Lines

cell name

NIH/3T3-Luc

Morphology

Fibroblast-like, adherent

Passage ratio

1:3~1:5

Species

Mouse

Reporter gene

Luciferase

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Accession

CVCL_C8Y9(Cellosaurus)

Luciferase validation

Sample	Compound pore 1	Compound pore 2	Compound pore 3	Average	Expressed multiple
NIH/3T3-Luc	158032	157843	185779	167218.0	182.022
NIH/3T3	952	987	817	918.667	182.022

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:NIH/3T3			STR Info (Cell bank) Cell Line:NIH-3T3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
4-2	233.16 【19.3】	237.36 【20.3】		19.3	20.3
5-5	335.38 【14】	339.45 【15】		14.0	15.0
6-4	290.24 【15.3】			15.3
6-7	334.14 【12】			12.0
9-2	220.72 【15】	224.74 【16】		15.0	16.0
12-1	241.32 【20】			20.0
15-3	192.53 【20.3】			20.3
18-3	155.35 【17】	163.49 【19】		17.0	19.0
X-1	399.83 【25】			25.0
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the NIH/3T3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NIH/3T3-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
01
Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
02
High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
03

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